Standardizing a simpler, more sensitive and accurate tail bleeding assay in mice

Figure 4 Tail bleeding time and volume measured in wild-type and FcRγ^-/- mice. Bleeding assay was performed by tail tip amputation and then immersing the tail in saline at 37°C. FcRγ^-/- mice showed a significant increase in both bleeding time and volume (P < 0.01 vs wild-type mice) and development of recurrent bleeding in 6/8 of animals studied. Note that the better separation of FcRγ^-/- and control mice by bleeding volume or index than that by bleeding time.