Copyright
©The Author(s) 2021.
World J Crit Care Med. Sep 9, 2021; 10(5): 170-182
Published online Sep 9, 2021. doi: 10.5492/wjccm.v10.i5.170
Published online Sep 9, 2021. doi: 10.5492/wjccm.v10.i5.170
The available models | Advantages | Disadvantages |
Monolayer cell models | Simplistic model; Easy to culture within short times | Cells underneath the medium, no connection to other types of cells and no tight junctions; Non-optimal physiologic response; The growth kinetics of bacteria, fungal or virus on monolayer are known to be different from human body |
Air liquid interface model | Polarized differentiated airway epithelium containing ciliated epithelial cells, basal cells and mucus producing cells, mimicking human epithelium; It can be co-cultured with pathogens; Respiratory virus is known to show similar replication kinetics as in human body | |
3D human lung spheroid model | 3D multicellular spheroids are small, tightly bound cellular aggregates that tend to form when cells are maintained under non-adherent conditions; Other cell types such as fibroblasts can be incorporated and can be co-cultured with pathogens | |
Animal models | In vivo | Have ethical issues and many results cannot be replicated in human studies; High cost; Time consuming, not applicable to high-throughput studies |
- Citation: Zhang YM. Orosomucoid-like protein 3, rhinovirus and asthma. World J Crit Care Med 2021; 10(5): 170-182
- URL: https://www.wjgnet.com/2220-3141/full/v10/i5/170.htm
- DOI: https://dx.doi.org/10.5492/wjccm.v10.i5.170