Regulatory T cells suppress autoreactive CD4+ T cell response to bladder epithelial antigen

Figure 8 Treg cells from urothelium-ovalbumin^GFP-Foxp3/OT-II mice are suppressive to ovalbumin-specific CD4⁺ T cells. A: OT-II splenocytes were incubated alone or in the presence of OVA323-339 peptide (10 μg/mL), GFP-positive (Foxp3⁺) CD4⁺ T cells (at a 1:1 ratio), and/or GFP-negative (Foxp3^-) CD4⁺ T cells (at a 1:1 ratio) sorted from URO-OVA^GFP-Foxp3/OT-II mice for 3 d. Proliferation was assessed by labeling the cultures with 3H-thymidine for the final 18 h. Data are presented as the mean ± SD from triplicate cultures. ^bP < 0.001 compared with OT-II cells stimulated with OVA_323-339 peptide alone (two-tailed Student’s t test); B: Culture supernatants from a parallel plate were collected after 3-d incubation and analyzed for IFN-γ by ELISA. Data are presented as the mean ± SD from duplicate cultures. ^aP < 0.05 compared with OT-II cells stimulated with OVA_323-339 peptide alone (two-tailed Student’s t test). URO^GFP-Foxp3/OT-II: Urothelium-ovalbumin^GFP-Foxp3/OT-II mice.