Regulatory T cells suppress autoreactive CD4+ T cell response to bladder epithelial antigen

Figure 5 OT-II CD4⁺ T cells infiltrate into the bladders of urothelium-ovalbumin/OT-II mice. Bladder single-cell suspensions were prepared from URO-OVA/OT-II mice (8 wk) and analyzed for surface CD4, Vα2 and Vβ5 by flow cytometry. Age-matched B6/OT-II mice were included for comparison. Gate was set on lymphocytes according to scatter criteria. Total numbers of CD4⁺, Vα2⁺ and Vβ5⁺ cells per bladder are indicated in (A) and percentages of single- and double-positive cells per bladder indicated in (B). Results are representative of 3 separate experiments consisting of 5-8 mice per group. URO/OT-II: Urothelium-ovalbumin/OT-II mice.