Regulatory T cells suppress autoreactive CD4+ T cell response to bladder epithelial antigen

Figure 3 Deletion-escaped OT-II CD4⁺ T cells retain responsiveness to ovalbumin. Splenocytes from URO-OVA/OT-II mice (8 wk) were cultured alone or in the presence of OVA_257-264 peptide (10 μg/mL) or OVA_323-339 peptide (10 μg/mL) for 3 d, followed by ELISA analysis of IFN-γ production in culture supernatants. Splenocytes from age-matched OT-II and B6/OT-II mice were included for comparison. Data are presented as the mean ± SD from duplicate determinations. ^bP < 0.001 compared with non-stimulated or OVA_257-264-stimulated splenocytes (two-tailed Student’s t test). URO/OT-II: Urothelium-ovalbumin/OT-II mice.