Regulatory T cells suppress autoreactive CD4+ T cell response to bladder epithelial antigen

Figure 2 Clonal deletion of OT-II CD4⁺ T cells in urothelium-ovalbumin/OT-II mice. Cells from the thymus (top panel), spleen (middle panel), and BLNs (bottom panel) of URO-OVA/OT-II mice (8 wk) were analyzed for surface CD4, Vα2 and Vβ5 by flow cytometry. Age-matched B6/OT-II mice were included for comparison. Gate was set on lymphocytes according to scatter criteria. Percentages of single- and double-positive cells are indicated. Results are representative of 3 separate experiments consisting of 4-6 mice per group. URO/OT-II: Urothelium-ovalbumin/OT-II mice.