Intracellular localisation of Mycobacterium marinum in mast cells

Figure 4 quantitative reverse transcriptase- polymerase chain reaction analysis of inflammatory genes from human mast cell line incubated with Mycobacterium marinum (MOI 10). The target inflammatory genes were normalised to individual housekeeping gene (β2M), thereafter the target genes were calculated against control human mast cell line cells and expressed as fold change. The error bars show standard deviation of two-four independent experiments, each in either duplicate or triplicate. NOD2: Nucleotide-binding oligomerization domain-containing protein 2; COX2: Cyclooxygenase 2; TNF-α: Tumour necrosis factor-α.