RNA polymerases in plasma cells trav-ELL2 the beat of a different drum

Figure 5 Igh gene has a different pattern of H3K7methylation in B cells vs antibody secreting cells. Distribution of histone H3K7me is enhanced in the region downstream of the internal Igh enhancer in plasma cells (ASCs). The 11 kb Igh gamma 2a gene is identical in the B and PC (hybridoma) lines and located in the intact Igh locus. A: Location of probes used in QPCRs. Cells were fixed and chromatin IP performed with the indicated antibodies specific to the individual K79 methylations; B: The B cell line A20; C: The plasma/hybridoma line AxJ which is an ASC. ASCs: Antibody secreting cells; QPCR: Quantitiative polymerase chain reaction; IP: Immunoprecipitation.