Eotaxin-2 blockade ameliorates experimental autoimmune encephalomyelitis

Figure 3 Anti-eo-2 neutralizing mAb treatment effect on pro-inflammatory cytokines profile. Experimental autoimmune encephalomyelitis (EAE) mice sera from total IgG and 100 μg D8 groups were assessed for the presence of interleukin (IL)-6, interferon (IFN)-γ, tumor necrosis factor (TNF)-α, IL-12p70 and macrophage chemoattractant protein (MCP)-1 using the BD™ Cytometric Bead Array Mouse Inflammation Kit. Treatment of EAE-induced mice with D8 100 μg led to a significant decrease of 57.3% in serum levels of MCP-1, 61.2% in serum levels of IFN-γ and 60.8% in levels of TNF-α, compared to IgG treatment (n = 6 in each group, ¹P = 0.03, ²P = 0.02, ³P = 0.005, two-tailed Student’s t-test).