Dynamic interplay of T helpercell subsets in experimental autoimmune encephalomyelitis

Figure 7 Analysis of effector and memory T cells in mice with experimental autoimmune encephalomyelitis. Spleen cells were isolated from C57BL/6 mice on different days following induction of experimental autoimmune encephalomyelitis. Fresh spleen cells were analyzed for surface antigens CD4, CD44 and CD127 by flow cytometry. A-E: Representative dot plots show CD44 and CD4 expression in CD127⁺ T cells at different time points; F: The percentages of CD4⁺CD44^lowCD127⁺ effector cells or G, CD4⁺CD44^highCD127⁺ memory T cells are shown for each time point. The graphs represent the mean ± SE from two independent experiments and the statistical significance is shown as ^aP < 0.05, ^bP < 0.01 vs naïve.