Dynamic interplay of T helpercell subsets in experimental autoimmune encephalomyelitis

Figure 5 Analysis of regulatory T cells in mice with experimental autoimmune encephalomyelitis. Spleen cells were isolated from C57BL/6 mice on different days following induction of experimental autoimmune encephalomyelitis. Fresh spleen cells were analyzed for surface antigens CD4 and CD25 and intracellular Foxp3 by flow cytometry. A-E: Representative dot plots show CD25 and Foxp3 expression in CD4⁺ T cells at different time points; F: The percentages of CD4⁺CD25⁺Foxp3⁺Tregs are shown for each time point and the graph represents the mean ± SE from two independent experiments. The statistical significance is shown as ^aP < 0.05.