Dynamic interplay of T helpercell subsets in experimental autoimmune encephalomyelitis

Figure 4 Analysis of T cell activation markers in mice with experimental autoimmune encephalomyelitis. Spleen cells were isolated from C57BL/6 mice on different days following induction of experimental autoimmune encephalomyelitis. A-B: Cells were cultured with phorbol 12-myristate 13-acetate and ionomycin for 6 h. GolgiStop (monensin) was added for the last 5 h of culture, and intracellular interleukin (IL)-17 and interferon gamma (IFNγ) were analyzed by flow cytometry; C: Fresh spleen cells were analyzed for expression of CD25; D: Foxp3; E: CD4; F: CD8; G: CD127; H: Related orphan receptor gamma (RORγ) by flow cytometry. The graphs show positive cells for selected single stain at each time point and represent the mean ± SE from at least two independent experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001.