Dynamic interplay of T helpercell subsets in experimental autoimmune encephalomyelitis

Figure 3 Secretion of cytokines from neural antigen sensitized spleen cells in culture. C57BL/6 male mice were induced to develop experimental autoimmune encephalomyelitis (EAE) by immunization with MOGp35-55. A: The clinical signs were scored on the day of sacrifice and presented as the mean ± SE for each group. The data represent 2 separate experiments; B-F: Spleen cells were isolated on different days following induction of EAE and cultured in the presence of 0 or 5 μg/mL of MOGp35-55 for 48 h. Cytokine secretion was analyzed in the pooled culture supernatants from two mice per group by enzyme linked immunosorbant assay. The data shown are the mean ± SE of triplicates.