Dynamic interplay of T helpercell subsets in experimental autoimmune encephalomyelitis

Figure 2 Expression of cytokines and transcription factors in the lymphoid organ of mice with experimental autoimmune encephalomyelitis. Spleen samples were isolated from C57BL/6 mice on different days as indicated following induction of experimental autoimmune encephalomyelitis (EAE). Total RNA was extracted from individual tissue samples and cDNA was reverse transcribed. Equal quantities of cDNA from two mice per group were combined and analyzed by quantitative reverse transcription polymerase chain reaction using 18S as the internal control. The fold changes in the expression of selected genes in the spleen of mice with EAE were calculated based on naïve as control. The data shown are the mean ± SE of quadruplicates and the statistical significance is shown as ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. ND: Not detectable.