Dynamic interplay of T helpercell subsets in experimental autoimmune encephalomyelitis

Figure 1 Expression of cytokines and transcription factors in the central nervous system of mice with experimental autoimmune encephalomyelitis. Brain samples were isolated from C57BL/6 mice on different days as indicated following induction of experimental autoimmune encephalomyelitis (EAE). Total RNA was extracted from individual tissue samples and reverse transcribed into cDNA. Equal quantities of cDNA from two mice per group were combined and analyzed by quantitative reverse transcription polymerase chain reaction using 18S as the internal control. The fold changes in the expression of selected genes in the central nervous system of mice with EAE were calculated based on naïve as control. ¹If naïve samples were undetectable, fold changes in the expression of cytokines in EAE mice were calculated based on day 14 samples as control. The data shown are the mean ± SE of quadruplicates and the statistical significance is shown as ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, vs naïve or day 14. ND: Not detectable; IFNγ: Interferon gamma; IL: Interleukin; RORγ: Related orphan receptor gamma.