Variation in experimental autoimmune encephalomyelitis scores in a mouse model of multiple sclerosis

Table 1 Commonly used classical experimental autoimmune encephalomyelitis score

Score	EAE signs	Observation
0	No signs of EAE	Hind legs are extended; tail extends up and moves; tail wraps around cylindrical object; normal gait
0.5	Partially limp tail	Hind legs are extended; tip of tail droops and/or does not wrap around cylindrical object; normal gait
1	Paralyzed tail	Hind legs are extended; tail droops and does not wrap around cylindrical object; normal gait
2	Loss in coordinated movement; hind limb paralysis	Hind legs contract when held at the base of tail; mouse walks with uncoordinated movement; hind limbs reflex when toes are pinched; limp tail
2.5	One hind limb paralysis	Mouse drags one hind limb; one hind limb does not respond to pinch; limp tail
3	Both hind limbs paralysis	Mouse drags both hind limbs; both hind limbs do not respond to toe pinch; limp tail
3.5	Hind limb paralysis; weakness in forelimbs	Mouse drags hind limbs but has difficulty using forelimbs to pull body; forelimbs respond to toe pinch; limp tail
4	Forelimbs paralysis	Mouse cannot move; forelimbs do not respond to toe pinch; limp tail
5	Moribund	No movement; cold to the touch; altered breathing

Note there are no scores denoted 1.5 and 4.5. The data available from reference 10. EAE: Experimental autoimmune encephalomyelitis.