Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

Figure 3 Exercise promotes osteogenic differentiation of bone mesenchymal stem cells through upregulation of lncRNA H19/miR-149. A: Alkaline phase staining of bone mesenchymal stem cells (BMSCs) 3 d after osteogenic induction of differentiation; B: Alizarin red staining of BMSCs 3 d after osteogenic induction of differentiation; C: Gene expression changes of lncRNA H19, miR-149 and osteogenic differentiation-related markers in BMSCs after osteogenic differentiation induction and si-lncRNA H19 interference by RT-qPCR; D and E: Protein expression changes of osteogenic differentiation-associated markers and autophagy-associated markers in BMSCs after osteogenic differentiation induction and si-lncRNA H19 interference by WB technique. n = 3, ^aP < 0.05, ^bP < 0.01. Con: Control group; NC: Negative control group; OI: Osteogenic differentiation induction group.