Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

Figure 2 Exercise promotes osteogenic differentiation of mouse bone mesenchymal stem cells. A: Alkaline phase staining of mouse bone mesenchymal stem cells (BMSCs) at 7 d of osteogenic differentiation induction; B: Alizarin red staining of mouse BMSCs at 14 d of osteogenic differentiation induction; C and D: Identification of potential miRNAs targets of lncRNA H19 by bioinformatics analysis; E and F: Changes in mRNA levels of lncRNA H19, miR-149, and osteogenic differentiation-associated markers after induction of osteogenic differentiation in mouse primary BMSCs by RT-qPCR; G and H: Changes in protein level of osteogenic differentiation-related marker and autophagy-related marker after induction of osteogenic differentiation in mouse primary BMSCs. n = 3, ^aP < 0.05, ^bP < 0.01. Con: Control group; OI: Osteogenic differentiation induction; Ex: Treadmill exercise group.