Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

doi:10.5312/wjo.v15.i4.363

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Apr 18, 2024 (publication date) through Nov 24, 2024

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World Journal of Orthopedics

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2218-5836

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World J Orthop. Apr 18, 2024; 15(4): 363-378
Published online Apr 18, 2024. doi: 10.5312/wjo.v15.i4.363

Table 2 Primer sequences

Genes	Primer sequence (5'-3')
lncRNA H19	Forward: 5'- GCTCCACTGACCTTCTAAAC -3' Reverse: 5'- ACGATGTCTCCTTTGCTAAC -3'
miR-149	Forward: 5'- TGGCTCCGTATCTTCACTCC -3'
β-actin	Forward: 5'-CTGTCCCTGTATGCCTCTG-3' Reverse: 5'-ATGTCACGCACGATTTCC-3'
ALP	Forward: 5'-CCAACTCTTTTGTGCCAGAGA-3' Reverse: 5'-GGCTACATTGGTGTTGAGCTTTT-3'
Runx2	Forward: 5'-AGAGTCAGATTACAGATCCCAGG-3' Reverse: 5'-TGGCTCTTCTTACTGAGAGAGG-3'
BMP2	Forward: 5'- GTGCAGATCCTAGGTTTCTCTG-3' Reverse: 5’- CAGGATCTCATTCTCTTGGATC-3’
Osterix	Forward: 5'-TGGTACAAGGCAGGCATCCA-3' Reverse: 5'-GGAGCAAAGTCAGATGGGTAAGT-3'

Citation: Zhou XC, Wang DX, Zhang CY, Yang YJ, Zhao RB, Liu SY, Ni GX. Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis. World J Orthop 2024; 15(4): 363-378
URL: https://www.wjgnet.com/2218-5836/full/v15/i4/363.htm
DOI: https://dx.doi.org/10.5312/wjo.v15.i4.363