Metagenomic next-generation sequencing may assist diagnosis of osteomyelitis caused by Mycobacterium houstonense: A case report

Figure 3 Cultivation of nontuberculous mycobacteria and microscopic images. A: Positive acid fast staining of pus, with a large number of red elongated hyphae (100 ×); B: Under the microscope, slender, red, rod-shaped nontuberculous mycobacteria could be seen, with relatively scattered distribution and some bacterial cells slightly curved (400 ×); C: On the 12^th day of observation, a unique scene appeared on Roche medium. Rice yellowish dry bacterial colonies appeared on top. This rice yellow color was very eye-catching and stood out, particularly against the background of the culture medium. The colony appeared dry and lacked a moist luster.