Basic Study
Copyright ©The Author(s) 2024.
World J Orthop. Nov 18, 2024; 15(11): 1075-1087
Published online Nov 18, 2024. doi: 10.5312/wjo.v15.i11.1075
Table 1 Primers in the 5´-3´ sequence for assessment of gene expression by real time-PCR
Name of the animal's mRNA
Sense
Antisense
IL-1βCCTATGTCTTGCCCGTGGAGCACACACTAGCAGGTCGTCA
IL-6CACTTCACAAGTCGGAGGCTTCTGACAGTGCATCATCGCT
IL-10GAAGGACCAGCTGGACAACAGGGGCATCACTTCTACCAGG
TNF-αATGGGCTCCCTCTCATCAGTGCTTGGTGGTTTGCTACGAC
RANKCCCTTGCAGCTCAACGTGGATAGTGCTTCCATCTTCCCAAGGA
RANKLCCTGTACTTTCGAGCGCAGAAGTCGAGTCCTGCAAACCTG
MMP-13ACCCAGCCCTATCCCTTGATTCTCGGGATGGATGCTCGTA
OPGCAACCGAGTGTGCGAATGTGTTGCAAACCGTGTTTCGCTC
MMP-1CCCAAATCCCATCCAGCCAAATTGAGCTCAGCTTCTGGCAT
MMP-8ACCCAATGGAATCCTTGCCCGGGTACATCAAGGCACCAGG
MMP-2AAGGATGGAGGCACGATTGGCCTTGGGGCAGCCATAGAAA