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©The Author(s) 2024.
World J Orthop. Oct 18, 2024; 15(10): 918-931
Published online Oct 18, 2024. doi: 10.5312/wjo.v15.i10.918
Published online Oct 18, 2024. doi: 10.5312/wjo.v15.i10.918
Figure 1 Schematic diagram of obtaining urine-derived stem cells and their morphology.
After collecting urine samples, cell precipitation is obtained by low-speed centrifugation. Then cells are resuspended with specific culture medium and inoculated in a 24-well plate. Next, appropriate cell clone spheres are selected and inoculated in a 6-well plate. Finally, spheres are identified and sub-cultivated. Urine-derived stem cells (USCs) can present as the shapes of rice, spindle, circle, and pebble.
- Citation: Yang HS, Zheng YX, Bai X, He XY, Wang TH. Application prospects of urine-derived stem cells in neurological and musculoskeletal diseases. World J Orthop 2024; 15(10): 918-931
- URL: https://www.wjgnet.com/2218-5836/full/v15/i10/918.htm
- DOI: https://dx.doi.org/10.5312/wjo.v15.i10.918