Combination immunotherapy with Survivin and luteinizing hormone-releasing hormone fusion protein in murine breast cancer model

Figure 5 Schematic representation of the study of anti-tumor effect of Survivin, luteinizing hormone-releasing hormone fusion protein individually and in combination. The study was conducted on four groups of mice: Untreated or control, Survivin alone, LHRH(6leu)LTB alone and Survivin + LHRH(6leu)LTB. Individual recombinant proteins were purified and used as immunogen along with Mycobacterium indicus pranii as an immunomodulator in murine model. The mice from all the groups were further challenged with tumor cells, 4T1 and tumor development was observed in each group. LHRH fusion protein was not effective in suppressing tumor alone. However, the combination of both Survivin and LHRH(6leu)LTB inhibited tumor growth substantially followed by Survivin alone. LHRH: Luteinizing hormone-releasing hormone.