Granulocyte colony-stimulating factor-producing hepatocellular carcinoma with abrupt changes

Figure 3 Histopathologic findings. Microscopic findings showed atypical poorly differentiated cells with a sheet structure (A); HCC tumor was also composed of sarcomatous spindle-shaped cells (B); in both samples, a drastic infiltration of the neutrophils was found (H and E, × 20). Immunohistochemical findings showed CAM5.2 positive in the moderately to poorly differentiated HCC lesion (C) and negative in the spindle-shaped cell lesion (D) (CAM5.2, × 20). Immunohistochemical examination showed that G-CSF was positive in the moderately to poorly differentiated HCC lesion (E, F) (G-CSF, × 20 and × 40). G-CSF: Granulocyte colony-stimulating factor; HCC: Hepatocellular carcinoma cell.