Fluoxetine induces cytotoxic endoplasmic reticulum stress and autophagy in triple negative breast cancer

Figure 1 Expression levels of proteins along the phosphoinositide 3-kinase/Akt/mammalian target of rapamcyin, mitogen-activated protein kinase/extracellular signal-regulated kinase, and apoptosis pathways vary by cell lines. Statistically significant and differentially expressed proteins, following (A) 24 h and (B) 48 h treatment of 10 μmol/L FLX, were indicated with stars; (C) Expression of few selected proteins across cell lines after 48 h of 10 μmol/L fluoxetine was confirmed by Western blotting. For detection of p70 S6K and ERK1/2 activation, cell extracts were loaded at 100 μg and 30 μg per lane, respectively. MEK: Mitogen-activated protein kinase; ERK: Extracellular signal-regulated kinase; FLX: Fluoxetine; p70 S6K: p70 S6 Kinase; GSK3: Glycogen synthase kinase 3; eIF4G: Eukaryotic translation initiation factor 4G.