Monitoring adenoviral based gene delivery in rat glioma by molecular imaging

Figure 6 Analysis of transgene delivery to tumor cells by double labeling. Sections were double with hNIS/vWF or hNIS/EGFR. Sections stain with hNIS/vWF clearly shows some of the red hNIS positive cells also express green vWF positive markers [positive for endothelial progenitor cells (EPC)] indicating the administered EPCs. While hNIS/EGFR double staining clearly shows some of the red positive cells (hNIS positive cells) also express green EGFR positive markers indicating expression of transgenes in glioma cells. Prussian blue staining shows the iron positive cells at the corresponding sites.