Inhibition of N-Myc down regulated gene 1 in in vitro cultured human glioblastoma cells

Figure 2 Detection and experimental monitoring of hypoxia induced N-Myc down-regulated gene 1 in specimens of human brain cancer. The experimental monitoring approach includes (A) experimental approach detection of the experimental results. Here, the tumor cell lines are first cultivated in vitro and subsequently transfected with the N-Myc down-regulated gene 1 (NDRG1) short dsRNA oligonucleotides (siRNA) construct and treated with fixed O₂ concentration in the hypoxia chamber followed by the specimens extraction, quantification, quality control and molecular seperation of tumor cells specimens. Further protein or mRNA blotting and Hybridization with subsequent NDRG1 expression image detection and documentation take place. The experimental approach with these different stages are repeated at least three times to have statistical significant results that are necessary for evaluation of the experimental results (B), where first the films with the detected results are scanned followed by the detection of the genes expression (which is in the case NDRG1 gene and the house keeping genes or loading controls (β-actin and 18s RNA, respectively) measurement of intensities in the analyzed specimens, statistical analysis and evaluation of the obtained results.