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World J Clin Oncol. Jun 10, 2012; 3(6): 82-91
Published online Jun 10, 2012. doi: 10.5306/wjco.v3.i6.82
Published online Jun 10, 2012. doi: 10.5306/wjco.v3.i6.82
Figure 4 Representative carbonic anhydrase 9 flow cytometry with suspensions of known aerobic and hypoxic cell populations (0.
1% O2 for 24 h) or cytokine stimulation under both aeration conditions. Suspensions of hepatocellular carcinoma cell lines during hypoxia or cytokine stimulation [interleukin (IL)-1, IL-6, tumor necrosis factor-alpha (TNF-α) or transforming growth factor-beta (TGF-β)] were incubated with the anti-carbonic anhydrase 9 (CA9) antibody M75 and a fluorescein-isothiocyanate-conjugated secondary antibody.
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Citation: Kockar F, Yildrim H, Sagkan RI, Hagemann C, Soysal Y, Anacker J, Hamza AA, Vordermark D, Flentje M, Said HM. Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells
in vitro . World J Clin Oncol 2012; 3(6): 82-91 - URL: https://www.wjgnet.com/2218-4333/full/v3/i6/82.htm
- DOI: https://dx.doi.org/10.5306/wjco.v3.i6.82