Copyright
©2012 Baishideng Publishing Group Co.
World J Clin Oncol. Jun 10, 2012; 3(6): 82-91
Published online Jun 10, 2012. doi: 10.5306/wjco.v3.i6.82
Published online Jun 10, 2012. doi: 10.5306/wjco.v3.i6.82
Figure 1 Hypoxia-inducible factor-1 alpha mRNA expression level determination via semi-quantitative reverse transcription polymerase chain reaction in the human hepatocellular carcinoma cell line Hep3B under aerobic and hypoxic conditions (0.
1% O2 for 24 h) without or with cytokine stimulation (interleukin-1, interleukin-6, tumor necrosis factor-alpha or transforming growth factor-beta) under both aeration conditions examined. β-actin was used as a loading control. Bar graphs show band intensities after densitometric evaluation. Representative experiment of three different experiments. IL: Interleukin; TNF-α: Tumor necrosis factor-alpha; TGF-β: Transforming growth factor-beta; HIF-1α: Hypoxia-inducible factor-1 alpha.
-
Citation: Kockar F, Yildrim H, Sagkan RI, Hagemann C, Soysal Y, Anacker J, Hamza AA, Vordermark D, Flentje M, Said HM. Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells
in vitro . World J Clin Oncol 2012; 3(6): 82-91 - URL: https://www.wjgnet.com/2218-4333/full/v3/i6/82.htm
- DOI: https://dx.doi.org/10.5306/wjco.v3.i6.82