Dendritic cell vaccination in glioblastoma after fluorescence-guided resection

Figure 1 Proliferation results of patients UPN 1, UPN 2 and UPN 5. PRE-n: PBMC obtained prior to the corresponding vaccine administration (same day). PBMCs obtained before each vaccination were plated in 96-well plates at 2 x 10⁵ cells/well with 20.000 dendritic cells pulsed with lysate and matured for five days. Then, cells were pulsed with 0.5 Ci/well of [3H] thymidine in order to determine [3H] thymidine incorporation. Dates are reported as stimulation index (SI). The SI was calculated as the ratio of the response to tumor lysate or pulsed dendritic cells over the mean response in the absence of tumor material.