Candida albicans and colorectal cancer: A paradoxical role revealed through metabolite profiling and prognostic modeling

Figure 8 Candida albicans metabolic mixture interferes with extracellular ATP content, apoptosis and PCR detection of prognostic model gene expression in colorectal cancer cells. A: Detection of extracellular ATP content; B: Histogram depicting cell apoptosis rates; C: Apoptosis rate in HCT-116 cells assessed via flow cytometry; D: Apoptosis rate in HT-29 cells detected by flow cytometry; E: Apoptosis rate in NCM-460 cells evaluated by flow cytometry; F: Expression levels of FDFT1, TIMP1, GADD45B, LIME1, and EHD4 across different cell types measured using real-time quantitative PCR (RT-qPCR); G: RT-qPCR analysis of FDFT1, TIMP1, GADD45B, LIME1, and EHD4 expression in HCT116 cells treated with the Candida albicans (C. albicans) metabolite mixture; H: RT-qPCR analysis of FDFT1, TIMP1, GADD45B, LIME1, and EHD4 expression in HT29 cells treated with the C. albicans metabolite mixture; I: RT-qPCR analysis of FDFT1, TIMP1, GADD45B, LIME1, and EHD4 expression in NCM460 cells treated with the C. albicans metabolite mixture. (^aP < 0.05,^bP < 0.01, ^cP < 0.001).