Downregulation of huntingtin-associated protein 1 predicts poor prognosis in gastric cancer

Figure 1 Huntingtin-associated protein 1 expression in gastric cancer tissues. A: Western blot analysis of huntingtin-associated protein 1 (HAP1) expression in tumor tissues and normal adjacent mucosa; B: Relative HAP1 protein level in 20 matched pairs of gastric cancer tissues and adjacent mucosa. β-actin was the control. HAP1 expression was obviously lower in tumor tissues than in normal mucosa (P < 0.001); C: Quantitative polymerase chain reaction measurement of HAP1 mRNA in tumor tissues and normal adjacent mucosa; D: Relative HAP1 mRNA expression in 20 matched pairs of gastric cancer tissues and adjacent mucosa; mucosa from sample 1 was the reference. HAP1 was lower in tumor tissues than in normal mucosa (P < 0.001). Glyceraldehyde 3-phosphate dehydrogenase was used as a control. means ± SD are shown. HAP1: Huntingtin-associated protein 1; N: Normal adjacent mucosa; T: Tumor tissues.