Transmembrane channel-like 5 drives hepatocellular carcinoma progression by regulating epithelial-mesenchymal transition

Figure 3 Transmembrane channel-like 5 promotes cell invasion, migration and proliferation. A: MHCC97-LM3 were transfected with three short hairpin RNAs (shRNAs) lentivirus, and MHCC-97H were transfected with transmembrane channel-like 5 (TMC5) overexpression lentivirus, conformed by qRT-PCR and western blot (n = 3); B: Colony formation formation assay of MHCC97-LM3 was transfected with three shRNAs lentivirus, MHCC-97H were transfected with TMC5 overexpression lentivirus (n = 3), scale bar = 500 μm; C: Transwell invasion assay of MHCC97-LM3 was transfected with three shRNAs lentivirus, MHCC-97H was transfected with TMC5 overexpression lentivirus, scale bar = 500 μm (n = 3); D: Wound healing assay of MHCC97-LM3 was transfected with three shRNAs lentivirus, MHCC-97H was transfected with TMC5 overexpression lentivirus (n = 3), scale bar = 200 μm. ^aP < 0.05; ^bP < 0.01. TMC5: Transmembrane channel-like 5; shNC: Short hairpin negative control; shRNA: Short hairpin RNA; OV: Overexpression lentivirus.