Targeting STAT3 with SH-4-54 suppresses stemness and chemoresistance in cancer stem-like cells derived from colorectal cancer

Figure 5 SH-4-54 treatment sensitizes cancer stem cells against oxaplatin. A: After being culture with 5 or 10 μg/mL of oxaplatin (OXA), SH-4-54 was added for co-culture, and then cell apoptosis was analyzed by performing Annexin V-FITC/PI double staining, followed by a flow cytometry assay; B: Total protein was fractioned and performed to detect apoptotic-related hallmarkers, including PARP, cleaved-PARP, caspase 3, and cleaved caspase-3. ^aP < 0.05 vs 5 μg/mL of OXA group; ^bP < 0.05 vs 10 μg/mL of OXA group. OXA: Oxaplatin.