Targeting STAT3 with SH-4-54 suppresses stemness and chemoresistance in cancer stem-like cells derived from colorectal cancer

Figure 4 SH-4-54 treatment decreased malignancies in cancer stem cells derived from SW480 and LoVo. A: After being cultured in IC₃₀ concentration of SH-4-54 for 24 hours, cells were fixed by 4% paraformaldehyde and analyzed by performing flow cytometry to detect DNA content; B: After being co-cultured in soft agar medium containing IC₃₀ concentration of SH-4-54 for 12 days, formed colonies were imaged; C: After being cultured in IC₃₀ concentration of SH-4-54 for 48 hours, Transwell assay was performed and transferred cells to lower membrane in five random views were calculated. ^aP < 0.05 vs PCs group; ^bP < 0.05 vs cancer stem cells group.