Targeting STAT3 with SH-4-54 suppresses stemness and chemoresistance in cancer stem-like cells derived from colorectal cancer

Figure 3 SH-4-54 decreased stemness hallmarkers via inhibiting phosphorylation of STAT3. A: Cancer stem cells (CSCs) were cultured with SH-4-54 with addition Colievlin or not, for 24 hours, then IL-6, JAK2/STAT3 signaling and stemness hallmarkers were detected by performing Western blot; B: With the presence of SH-4-54 with or without Colievlin, spheroid formation was imaged after 12-day incubation; C: After addition of Colievlin, the stemness hallmarkers were detected by performing western blot, including ALDH1A1, CD44 and Nanog. ^aP < 0.05 vs CSCs group; ^bP < 0.05 vs CSCs+SH-4-54 group. PCs: Parental cells; CSCs: Cancer stem cells.