Targeting STAT3 with SH-4-54 suppresses stemness and chemoresistance in cancer stem-like cells derived from colorectal cancer

Figure 1 The effects of SH-4-45 on ALDH1A1-positive proportion of colorectal cancer cells. A: After being cultured with 1-10 μmol/L of SH-4-45 for 24 hours, cell viability was measured by performing CCK-8 assay; B: Western blot was performed to detect STAT3 phosphorylation; C: Flow cytometry assay was performed to detect ALDH1A1-positive proportion; D: After being cultured in SH-4-54 for 24 hours, cell viability was detected. CSCs: Cancer stem cells.