Preparation of kakkatin derivatives and their anti-tumor activity

Figure 3 The 6-(hept-6-yn-1-yloxy)-3-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one inhibited the proliferation, invasion and metastasis of hepatocellular carcinoma SMMC-7721 cells. A: Cloning assay evaluated the effect of 6-(hept-6-yn-1-yloxy)-3-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one (HK) on the proliferation of SMMC-7721 cells; B: Colony formation assay to quantify cell proliferation; C: Transwell assay to detect the inhibitory effect of HK on the invasion of SMMC-7721 cells; D: Transwell assay to quantify SMMC-7721 cells; E: Scratch assay to detect the inhibitory effect of HK on the lateral migration of SMMC-7721 cells; F: Scratch assay to quantify SMMC-7721 cells. ^aP < 0.05; ^bP < 0.01; ^cP < 0.05; ^dP < 0.01; ^eP < 0.05; ^fP < 0.01; ^gP < 0.05; ^hP < 0.01. CDDP: Cisplatin; HK: 6-(hept-6-yn-1-yloxy)-3-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one.