Tankyrase 2 promotes lung cancer cell malignancy

Figure 1 Successful construction of cancer cell lines with TNKS2 knockdown or overexpression. A: Real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) analysis of tankyrase 2 (TNKS2) mRNA expression in all four lung cancer cell lines; B and C: Dot-blot analysis of TNKS2 protein expression in all four lung cancer cell lines; D: Construction of stably transfected non-small cell lung cancer cell lines with TNKS2 interference and overexpression. Efficiency of TNKS2 interference or overexpression was assessed in H647 and A549 cells utilizing RT-qPCR; E: Efficiency of TNKS2 interference or overexpression was assessed in H647 and A549 cells via Western blot analysis. ^aP < 0.05; ^bP < 0.01.