Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma

Figure 4 Effect of TMEM100 overexpression on mitogen-activated protein kinase pathway activation in KYSE-150/KYSE-450 cells. A: K-150 cells were harvested 24 h after transfection with TMEM100-oe, and total proteins were extracted for western blotting analysis. Phosphorylated-extracellular regulated kinase (p-ERK) and ERK, phosphorylated-c-Jun N-terminal kinase (p-JNK) and JNK, and p-p38 and p38 were analysed. The result demonstrated a reduction in the expression of p-ERK, p-p38, and p-JNK in K-150/K-450 cells transfected with TMEM100-oe; B: The experiment was repeated again with K-450 cells. p-ERK: Phosphorylated-extracellular regulated kinase; p-JNK: Phosphorylated-c-Jun N-terminal kinase.