Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma

Figure 2 Overexpression effect of TMEM100 in esophageal squamous cell carcinoma lines and the inhibitory effect of TMEM100 overexpression on proliferation, migration, and invasion of esophageal squamous cell carcinoma cells in vitro. A and B: K-150/K-450 cells transfected with TMEM100-oe were assayed using real-time PCR and western blotting, and the results showed that the expression of TMEM100 was significantly upregulated in the transfected cells compared to that in the control group; C: Colony formation viability of K-150/K-450 cells after transient transfection treatment for 14 d was analysed by staining with 1% crystal violet; D: Cell counting kit-8 assay results show that overexpression of TMEM100 inhibits the proliferation of K-150/K-450 cells. ^cP < 0.0001.