Regulation of TMEM100 expression by epigenetic modification, effects on proliferation and invasion of esophageal squamous carcinoma

Figure 1 Relationship between low TMEM100 expression in esophageal cancer and patient survival and the effect of 5-AZA on TMEM100 expression in esophageal squamous cell carcinoma lines. A: Expression profile of TMEM100 in EC samples compared with normal samples, showing reduced expression of TMEM100 in EC tissues; B: Overall survival of patients with high vs low TMEM100 expression levels. Survival was poorer for those with low TMEM100 expression (P = 0.041); C: 5-AZA induced a dose-dependent expression of TMEM100 in K-150 cells. Real-time PCR and western blotting results showed that after 24 h of treatment, TMEM100 expression increased with increasing 5-AZA concentration. ^aP < 0.05, ^bP < 0.01. DMSO: Dimethyl sulfoxide; EC: Esophageal cancer; ESCA: Esophageal cancer; HR: Hazard Ratio.