Limonin inhibits the stemness of cancer stem-like cells derived from colorectal carcinoma cells potentially via blocking STAT3 signaling

Figure 6 Limonin inhibits STAT3 signaling. A: After being cultured using limonin, total STAT3, phosphorylated STAT3 (Y705) or STAT3 (S727), in cytoplasmic or nucleus fraction, were measured by performing western blot; B: Stemness hallmarkers, including Nanog and β-catenin, were detected by western blot after Limonin treatment with or without 2.5 μmol/L colievlin pretreatment; C: Sphere formation was observed after Limonin treatment with or without 2.5 μmol/L colievlin pretreatment. ^aP < 0.05 vs parental cells group; ^bP < 0.05 vs cancer stem-like cells group. PCs: Parental cells; CSCs: Cancer stem-like cells.