Limonin inhibits the stemness of cancer stem-like cells derived from colorectal carcinoma cells potentially via blocking STAT3 signaling

Figure 5 Limonin pre-treatment inhibits tumor formation in nude mice. A: Cancer stem-like cells pretreated using 20 μmol/L limonin or not were seeded in nude mice and be maintained for 30 d. From day 10, tumor size was measured every five days. ^aP < 0.05 vs Mock group; B: Hematoxylin and eosin staining was performed to detect physiological morphology; C: Ki67 was stained.