Limonin inhibits the stemness of cancer stem-like cells derived from colorectal carcinoma cells potentially via blocking STAT3 signaling

Figure 3 Limonin inhibits stemness in cancer stem-like cells derived from colorectal cancer cells. A: After being culture with 10-60 μmol/L limonin for 10 d in serum-free medium, spheroid formation was observed; B: 20 μmol/L limonin was employed for treatment, and stemness hallmarkers, including Nanog and β-catenin were measured by western blot; C: Aldehyde dehydrogenase 1-positive proportion was measured by flow cytometry. PC: Parental cells; CSCs: Cancer stem-like cells.