Limonin inhibits the stemness of cancer stem-like cells derived from colorectal carcinoma cells potentially via blocking STAT3 signaling

Figure 2 Limonin inhibits malignancies in colorectal cancer cells. A: After being cultured with 20 or 40 μmol/L limonin for 24 h without Matrigel, or for 48 h with Matrigel, cells transferred to lower membrane were fixed, stained and counted. ^aP < 0.05 vs Mock group; B: After being cultured with 20 or 40 μmol/L limonin for 14 d, colonies on plates or soft agar were fixed and stained.