Thymoquinone enhances the antioxidant and anticancer activity of Lebanese propolis

Figure 2 Invitro antihemolytic/cytoprotective activity of each of Thymoquinone and methanol propolis extract from Rashaya and Akkar-Danniyeh against H₂O₂- induced oxidative hemolysis. Human red blood cells suspension was preincubated with methanol propolis extract from Rashaya (MPE-R), methanol propolis extract from Akkar-Danniyeh (MPE-D), or Thymoquinone (TQ; 10-100 µg/mL) for 5 min. The cell suspension was then incubated with 10% H₂O₂ for 90 min at 37°C. The samples were then centrifuged, and the absorbance of the supernatant was measured. The values are expressed as percentage of decrease in hemolysis with respect to the positive control (10 % H₂O₂). Each value is obtained from n = 1 experiment performed in monoplicate.