Thymoquinone enhances the antioxidant and anticancer activity of Lebanese propolis

Figure 1 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity of methanol propolis extract alone or in combination with Thymoquinone. A: 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of each of methanol propolis extract from Rashaya (MPE-R) and from Akkar-Danniyeh (MPE-D; 20-100 µg/mL) alone; B: DPPH free radical scavenging activity of each of MPE-R and MPE-D (10-50 µg/mL) in combination with Thymoquinone (TQ; 12.5-100 µg/mL). The samples were mixed with DPPH and the absorbance of the mixture was measured after 30 min. The values are expressed as percentage of DPPH percentage inhibition relative to the control. Each value represents the mean ± SD of n = 2 experiments. ^aP < 0.05 and ^bP < 0.01 are significantly different from control using two-tailed Student's t-test.