Classification of patients with metastatic colorectal cancer into consensus molecular subtypes into real-world: A pilot study

Table 1 Primers employed for reverse transcription-quantitative polymerase chain reaction experiments to determine the expression of β-catenin, c-Myc and TFG-β, and the genes used as reference genes

Name	Primer	Sequence	Product Lenght	Ref.
TGF-β	Forward	5’- TACCTGAACCCGTGTTGCTCTC-3’	122	[10]
	Reverse	5’- GTTGCTGAGGTATCGCCAGGA-3’
β-catenin	Forward	5’- CACAAGCAGAGTGCTGAAGGTG-3’	146	[11]
	Reverse	5’- GATTCCTGAGAGTCCAAAGACAG-3’
c-MYC	Forward	5’-GCCACGTCTCCACACATCAG-3’	132	[12]
	Reverse	5’-TGGTGCATTTTCGGTTGTTG-3’
B2M	Forward	5’-GTGCTCGCGCTACTCTCTC-3’	150	[13]
	Reverse	5’-GTCAACTTCAATGTCGGAT-3’
PPIA	Forward	5’-GCAAATGCTGGACCCAACACAAAT-3’	174	[14]
	Reverse	5’-AATGGTGATCTTCTTGCTGGTCTTG-3’
RPLP0	Forward	5’-GCAATGTTGCCAGTGTCTG-3’	142	[15]
	Reverse	5’-GCCTTGACCTTTTCAGCAA-3’