Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors

Figure 3 In vitro phototoxicity of folate-Si-rhodamine-1 after laser irradiation. The cells were stained using the reagents from the Cell viability imaging kit at 2 h after near-infrared photodynamic therapy (NIR-PDT). The nuclei of dead cells were stained with NucGreen^® Dead reagent (green), while the viable cell nuclei were stained with NucBlue^® Live reagent (blue). Rapid cell death-induced by PDT (10 J/cm²) and abundant dead cells were clearly noted. (scale bar = 500 μm, 10 × magnification). NIR-PDT: Near-infrared photodynamic therapy; IR: Laser irradiation; CTR: Control; NucGreen: NucGreen^® Dead reagent; NucBlue: NucBlue^® Live reagent; PC: Phase-contrast; μM: μmol/L.